This list of Saint Leo University alumni includes graduates, non-graduate former students and current students of Saint Leo University and its predecessor, Saint Leo College Preparatory School which was closed 1964 when the institution's focus shifted to higher learning.
| Alumni | Notability |
|---|---|
| Desi Arnaz | Actor (St. Leo College Prep) |
| Red Barrett | Major league pitcher (St. Leo College Prep) |
| Jennifer Carroll | Lieutenant Governor-elect of Florida |
| Michael Cicconetti | Notable judge |
| Jim Corsi | Major league pitcher |
| Anastasio Somoza Debayle | Former President of Nicaragua (St. Leo College Prep) |
| Luis Somoza Debayle | Former President of Nicaragua (St. Leo College Prep) |
| Lee Marvin | Actor (St. Leo College Prep) |
| Rodney J. McKinley | Former Chief Master Sergeant of the Air Force |
| Sankar Montoute | NFL player |
| JP Ricciardi | Current Executive Vice President and General Manager for the Toronto Blue Jays |
| Shannon Schambeau | The Miss District of Columbia for 2005 |
| Stephen Stills | Musician (St. Leo College Prep) |
| Adam Anicich | Military and Management Theorist. |
| Bob Tewksbury | Major league pitcher |
| Andrew Sousa | Voted Strongest Man in the UK |